Vitamin Testing
Quantify fat- and water-soluble vitamins in food, beverages and supplements to validate fortification levels and RDA claims against FSSAI limits.
What is vitamin testing?
Vitamin testing quantifies fat-soluble vitamins (A, D, E, K) and water-soluble vitamins (B-complex, C) in food, beverages and supplements using HPLC-based methods. Fortified products increasingly compete on vitamin content: "100% RDA of Vitamin D," "fortified with B12." These claims are only defensible if the actual quantity in the product matches what's printed on the label.
Equinox Labs quantifies fat-soluble vitamins (A, D, E, K) and water-soluble vitamins (B-complex, C) in food, beverages and supplements using HPLC-based methods at NABL-accredited laboratories, so your fortification and RDA claims are backed by verifiable data. It's often paired with nutraceutical & supplements testing and nutritional labeling.
What happens when vitamin content isn't verified?
None of these show up until a lab test flags them.
- Under-fortification risk. Storage, processing or mixing losses can leave a product below its stated RDA claim.
- Over-fortification risk. FSSAI sets maximum thresholds too, and exceeding them is just as much a violation as falling short.
- Labelling penalties. A verified gap between label and formulation is grounds for an FSSAI notice or a retailer delisting.
- Eroded consumer trust. Fortification claims drive purchase decisions, and an unverifiable one damages the brand behind it.
What does vitamin testing check?
Both fat-soluble and water-soluble vitamin panels, in one submission.
Fat-Soluble Vitamins
Vitamin A, D, E and K quantification in oils, dairy, fortified staples and supplements.
Water-Soluble Vitamins
B-complex (B1, B2, B3, B6, B9, B12) and Vitamin C testing in beverages and functional foods.
Fortification & RDA Validation
Confirms fortified products meet FSSAI's minimum and maximum fortification thresholds.
Stability & Shelf-Life Cross-Check
Confirms vitamin content holds up across the claimed shelf life, not just at production.
Supplement Formulation Testing
Multivitamin and single-nutrient tablets, capsules and powders verified against label claims.
Regulatory Submission Reports
Structured reports formatted for FSSAI inspection or export documentation.
Which vitamins are tested?
The full panel used for label and fortification validation.
Fat-Soluble
Water-Soluble (B-Complex)
Other
Who needs vitamin testing?
From fortified staple manufacturers to supplement brands.
Fortified Food & Beverage Brands
Validate fortification levels for milk, atta, oil, salt and functional beverages.
Supplement Manufacturers
Confirm multivitamin and single-nutrient formulations match label claims.
Regulatory & QA Teams
Third-party validation ahead of an FSSAI inspection or label audit.
Infant & Toddler Nutrition Brands
Tight fortification tolerances make independent verification especially important here.
Exporters
Vitamin panels formatted to support international shipment and customs documentation.
Contract Manufacturers
Batch-level verification for private-label fortified products made on behalf of a brand.
Sample to certified report, in four steps
The same process across every vitamin testing engagement.
Inquire
Tell us which vitamins and product format need testing.
Sample Collection
Send in or have us collect samples from your production facility.
HPLC Testing
Samples are quantified using HPLC-based methods at our accredited labs.
Results & Support
We email your certified report and advise on label or formulation adjustments.
Access the Equinox PortalWhy choose Equinox Labs for vitamin testing?
Accredited HPLC methodology and regulatory fluency in fortification norms.
NABL Accredited
ISO/IEC 17025 accredited HPLC testing that regulators and retailers trust.
Fortification Standard Fluency
Familiar with FSSAI's minimum and maximum fortification thresholds.
Pan-India Reach
Sample logistics and testing coverage across 500+ cities, trusted by 95,000+ clients.
A Dedicated Account Manager
One point of contact for recurring batch testing.
Vitamin testing FAQs
Vitamin testing quantifies fat-soluble vitamins (A, D, E, K) and water-soluble vitamins (B-complex, C) in food, beverages and supplements using HPLC-based methods, to confirm the actual quantity matches what is printed on the label and meets FSSAI fortification thresholds.
We test fat-soluble vitamins A, D, E and K, and water-soluble B-complex vitamins (B1, B2, B3, B6, B9, B12) plus Vitamin C, across food, beverage and supplement matrices.
Common causes include vitamin degradation during storage or processing, inconsistent mixing during fortification, or the fortificant dose being set incorrectly at formulation stage.
We primarily use HPLC (High-Performance Liquid Chromatography), with LC-MS used for more complex or trace-level analysis where higher sensitivity is required.
Yes, we test individual vitamins or the full panel depending on what your label claim or regulatory submission requires.
FSSAI's fortification standards set both minimum and maximum thresholds for added vitamins. Falling outside either limit can trigger a labelling violation, which is why batch-level verification matters.
Yes. We test multivitamin and single-nutrient supplement formulations alongside fortified staples, beverages and functional foods, using the same HPLC-based methodology.
Get your Vitamin Testing enquiry answered
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